Introduction to Microbiology (CP4089) - Experiment 4: Colony Count Methods: Viable Cell Number of Commercial Active Dried Yeasts

Summary This purpose of this investigate was for students to do the liquidation recite methods, estimating the practicable cell event of commercial supple dry yeasts (ADY). This prove allowed the students to per progress to the headquarters deliberate technique by resultant dilution and two common methods, scattering musical scale and swarm plate to specialise the colonization forming unit (CFU) of yeasts A ten-fold dilution is usage of goods and servicesd in this experiment, the archetype distribution is diluted until it reached the 10-9 dilution. Plating for strewing plate started from 10-5, 10-6, 10-7 and 10-8 dilution while for pour plate, it started from 10-6, 10-7, 10-8 and 10-9 dilution. Having incubated inverted at agency temperature (25oC) for 2 days, the feasible cells for spread plate and pour plate were calculated. The CFU/g obtained for spread plate and pour plate were 2.2 X 1010 CFU/g and 3.7 X 1010.the CFU/g respectively. Introduct ion Colony count methodology is iodin of the most accurate methods to determine the unhurriedness of the organisms in a given sample. It enumerates the number of certain live, possible cells in the sample that form colonies on a fitted agar medium. As the optimum medium and conditions varies for one sample to another, the colony count methods provide an estimate of the number of viable cells according to the medium employed, time and temperature of incubation. Each colony that appears on the agar plate arising either from a flock of cells or from a single cell is referred as a colony forming unit (CFU). The sample used in this experiment is active dried yeasts (ADY).

A serial diluti on is performed to suspend the yeasts contai! ning ingathering in water so that the number of microorganisms per ml is low-spirited tolerable to be counted, when the sample is plated. Cells in overcrowded plates may not form colonies but may... Hi, Its regarding the results and calculation part. Theres some mistakes I think. For run the cfu/g for the spread plate and pour plate, the dilution number should use the same. Which fashion that both should use 10^-8, whr ^ means to the causation of. consequently if use the correct dilution number to count the cfu/g, the spread plates cfu is higher(prenominal) than the pour plates cfu, which tally with the theoretical CFU. If you want to ascertain a full essay, order it on our website: OrderEssay.net

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